Diagnostic Tumor
Genome Analysis (Next-Generation Sequencing, NGS)
Related Information (german)
Molecular Diagnostics using resections, biopsies, or cytological smears. The analysis includes the detection of mutations using DNA and/or translocations (rearrangements / fusiontranscripts) using RNA. NGS allows the analysis of multiple genes in parallel and is a multiplex-PCR similar to Sanger Sequencing. The detection of translocations by NGS is similar to fluorescence in-situ hybridization (FISH).
The majority of the analyses can be performed using either fixed or fresh material (i.e., FFPE, EDTA-blood, frozen tissue, FNP, unfixed tissue (Direction PDF).
Form for the Diagnostic Tumor
Genome Analysis
Order
form to fill out and print
General
informed consent form for research
Panel description
Mutationanalysis fast-track (Results within 24 - 48h):
Idylla™ NRAS-BRAF Mutations Test (Flyer)
Idylla™ KRAS Mutations Test (Flyer)
Idylla™ EGFR Mutations Test (Flyer)
Systematic search for Drug Targets in solid tumors (mutations, amplifications, gen fusions):
Oncomine Focus Assay™ Panel (DNA, RNA) -> gene list (XLS |
PDF)
Liquid Biopsy (Analyses of circulating tumor DNA in plasma):
Oncomine Focus Assay™ Panel (DNA) -> gene list (XLS |
PDF)
Therapy stratification
of patients with ovarian cancer (all coding exons):
Oncomine™
BRCA Research Assay (Flyer)
Therapy stratification of patients with CLL (all coding exons)
Ion AmpliSeq™
TP53 Panel
Often mutated gens (54) in patients with
myeloid diseases (e.g. AML):
TruSight
Myeloid® Sequencing Panel (Flyer) -> gene list (XLS |
PDF)
Often translocated gens (26) in patients with sarcomas
Archer Sarcoma Panel (Flyer) --> gene list translocation ( XLS | PDF )
Information NGS
ngs.pathologie@usz.ch
Phone +41 44 255 39 29
Dr. rer. nat. Nadejda Valtcheva
nadejda.valtcheva@usz.ch
Dr. rer. nat. Christine Fritz
christine.fritz@usz.ch
Dr. sc. nat. Sandra Freiberger
sandra.freiberger@usz.ch
Contact address laboratory
Dr. sc. nat. Markus Rechsteiner
Phone +41 44 255 39 29
markus.rechsteiner@usz.ch
